Smad2/3a对斑马鱼神经嵴细胞标记基因crestin表达的影响

论文摘要

目的探讨Smad2/3a对脊椎动物神经嵴细胞发育的影响。方法通过在斑马鱼胚胎单细胞时期显微注射smad2/3吗啉环修饰的反义寡核苷酸的方法,特异性敲降smad2/3基因的表达,至胚胎发育至6体节,利用整胚原位杂交检测神经嵴细胞特异性标记基因snail1b,sox10,foxd3和crestin的表达情况;通过casmad2 mRNA和smad3a mRNA显微注射的方法过表达smad2和smad3a,同样利用整胚原位杂交检测神经嵴细胞特异性标记基因crestin的表达情况;通过过表达casmad2及smad3a对下调smad2和smad3a的胚胎进行挽救。结果 smad2/3a被敲低后,crestin的表达量显著降低,而snail1b,sox10和foxd3的表达量无明显变化。smad3b被敲低后,crestin,snail1b,sox10和foxd3的表达量均无明显变化;过表达casmad2和smad3a均可导致crestin的表达量增高;过表达casmad2和smad3a可挽救由于smad2/3a敲降所造成crestin的低表达量。结论 Smad2和Smad3a对神经嵴细胞标记基因crestin的表达具有重要作用。

论文目录

文章来源

类型: 期刊论文

作者: 李丽芳,陈露西,贾顺姬,颜光玗

关键词: 斑马鱼,神经嵴细胞

来源: 中国实验动物学报 2019年04期

年度: 2019

分类: 基础科学,医药卫生科技

专业: 生物学

单位: 南方医科大学基础医学院发育生物学教研室,清华大学生命科学学院膜生物学国家重点实验室

基金: 国家自然科学基金面上项目(31522035,31371460,31522031,31571526)~~

分类号: Q75

页码: 415-422

总页数: 8

文件大小: 688K

下载量: 129

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