二株Streptomyces diastaticus活性代谢产物及其抑制病原菌机制初探

论文摘要

放线菌是微生物中一类重要的菌群,其次级代谢产物结构类型丰富,具有抗细菌、抗真菌、抗肿瘤、免疫抑制剂等多种生物活性,是微生物药物的重要来源。本论文通过对17株放线菌进行筛选,得到了具有良好抑菌活性且代谢产物化学成分丰富的两株放线菌菌株YIM PH20095和YIM PH20246,进行后续次生代谢产物及抗真菌活性机制研究。通过16S rRNA基因序列分析,两株放线菌均归属为Streptomyces diastaticus subsp.ardesiacus。采用色谱分离技术,从菌株YIM PH20095的发酵上清液中得到12个化合物,通过质谱和核磁共振数据,确定它们的结构分别为:8-hydroxyphenazine-1-carboxylic acid、2,5-piperazinedione,6-[（4-hydroxyphenyl）methylene]-1-methyl-3-phenylmethylene、newphenazine A、newphenazine B、9-methoxyphenazine-1-carboxylic acid、methyl benzoate、newphenazine C、1-methoxyphenazine、1,6-phenazinedicarboxylic acid、newphenazine D、newphenazine E和newphenazine F,其中化合物newphenazine A-F是未见文献报道的新化合物。从菌株YIM PH20246的发酵上清液中分离纯化得到7个化合物,通过质谱和核磁共振数据,确定它们的结构分别为:1-hydroxyphenazine、newphenazine G、6-carbamoylphenazine-1-carboxylic acid、newphenazine H、7-hydroxy-6（methoxycarbonyl）phenazine-1-carboxylic acid、phenazine-1-carboxamide和6-hydroxyphenazine-1-carboxylic acid,其中化合物newphenazine G-H是未见文献报道的新化合物。以4株三七根腐病原真菌及5株常见病原细菌为指示菌,测定了所得单体化合物的抑菌活性。19个单体化合物对测试病原菌具有不同程度的抑菌活性,其MIC值的范围在8-512 μg/mL之间。此外,采用生长速率法和孢子萌发法,对菌株YIMPH20095的粗提物拮抗黄瓜萎蔫病原真菌的作用机制进行了初步研究。

论文目录

文章来源

类型: 硕士论文

作者: 胡林芳

导师: 李一青

关键词: 放线菌,次生代谢产物,分离纯化,抑菌活性

来源: 云南大学

年度: 2019

分类: 基础科学

专业: 生物学

单位: 云南大学

基金: 国家自然科学基金“三七根际土壤放线菌抗根腐病原菌活性次生代谢产物研究”(基金号:31660532)

分类号: Q939.13

总页数: 98

文件大小: 6565K

下载量: 27

相关论文文献

• [1].1,2-Dibromoethane and KI mediated α-acyloxylation of ketones with carboxylic acids[J]. Chinese Chemical Letters 2020(03)
• [2].Dichloromethane as a methylene synthon for regioselective linkage of diverse carboxylic acids:Direct access to methylene diesters under metal-free conditions[J]. Chinese Chemical Letters 2019(06)
• [3].Synthesis,Configuration Judgment and Potentiation Activity of (2S,3S,E)-4-(3,4-Dihydroxybenzylidene)-3-(3,4-dihydroxyphenyl)-5-oxotetrahydrofuran-2-carboxylic Acid[J]. 结构化学 2017(09)
• [4].Significantly improved oxidation of bio-based furans into furan carboxylic acids using substrate-adapted whole cells[J]. Journal of Energy Chemistry 2020(02)
• [5].Mild oxidative degradation of spent auricularia auricular substrate and molecular composition of carboxylic acids in the resulting soluble portion[J]. Chinese Journal of Chemical Engineering 2020(02)
• [6].Crystal Structure, Electrochemical and Fluorescent Properties of a New Copper(Ⅱ) Coordination Polymer with 2,3-Pyridinedi-carboxylic Acid[J]. Chinese Journal of Structural Chemistry 2019(11)
• [7].How Effective Is the Single-point Energy Calculation in Investigating the Reaction Mechanisms?New Decarboxylation Mechanism of Pyrrole-2-carboxylic Acid by Full Optimization with CPCM Solvation Model[J]. 结构化学 2013(10)
• [8].Electrocarboxylation of Anthrone to Anthracene-9-carboxylic Acid in the Presence of CO_2[J]. Chemical Research in Chinese Universities 2011(06)
• [9].Synthesis of 6-sulfamoyl-4-oxoquinoline-3-carboxylic acid derivatives as integrase antagonists with anti-HIV activity[J]. Chinese Chemical Letters 2009(07)
• [10].Synthesis and Characterization of Novel Fluoroalkyl Unsaturated Multi-carboxylic Acid Esters[J]. Journal of Donghua University(English Edition) 2012(03)
• [11].Synthesis and Crystal Structure of 2-Fluoro-4-methyl-3-oxo-4-aza-5α-androst-1-ene-17β-carboxylic Acid Methyl Ester[J]. 结构化学 2010(04)
• [12].Preparation of ferrocenyl imidazolines using bis(triphenyl)oxodiphosphonium trifluoromethanesulfonate[J]. Science in China(Series B:Chemistry) 2009(09)
• [13].Pyrazole-3-carboxylic Acid Manganese Complex: Syntheses and Its highly Efficient Heterogeneous Catalysis for the Epoxidation of Alkenes[J]. Chinese Journal of Structural Chemistry 2019(04)
• [14].Crystal Structure and Properties of [PrCl(H_2O)_3(L)(HL)]_nnCl(HL=3-Hydroxy-2-methylquinoline-4-carboxylic acid) with One-dimensional Chains[J]. Chinese Journal of Structural Chemistry 2019(02)
• [15].Synthesis and biological activities of novel 2-amino-1,3-thiazole-4-carboxylic acid derivatives[J]. Chinese Chemical Letters 2015(10)
• [16].Synthesis and Crystal Structure of a Luminescent Compound 5-(1H-benzoimidazol-2-yl)-3H-[1,2,3]triazole-4-carboxylic Acid[J]. 结构化学 2012(11)
• [17].A Novel Palladium(Ⅱ)Complex Self-assembled from Anthracene-9-carboxylic Acid:Synthesis,Crystal Structure and Spectroscopic Properties[J]. 结构化学 2013(10)
• [18].One-pot,direct synthesis of acyl azides from carboxylic acids using Ph_2PCl/I_2/NaN_3 reagent system[J]. Chinese Chemical Letters 2012(04)
• [19].Two Copper Complexes Based on Pyrazole-3-carboxylic Acid as Heterogeneous Catalysts for Highly Selective Oxidation of Alkylbenzenes[J]. 结构化学 2018(02)
• [20].Decarboxylative bromination of α,β-unsaturated carboxylic acids via an anodic oxidation[J]. Chinese Chemical Letters 2017(06)
• [21].Seasonal variations of carboxylic acids and their contributions to the rainwater acidity: A case study of Guiyang and Shangzhong,China[J]. Chinese Science Bulletin 2010(16)
• [22].Precipitation transformation of rare earth sulfate into chloride with p-dodecylphenoxy carboxylic acids[J]. Journal of Rare Earths 2019(12)
• [23].Density functional theory study of the mechanism of the acid-catalyzed decarboxylation of pyrrole-2-carboxylic acid and mesitoic acid[J]. Science China(Chemistry) 2011(05)
• [24].QSAR and Docking Studies of Thiazolidine-4-carboxylic Acid Derivatives as Neuraminidase Inhibitors[J]. Chinese Journal of Structural Chemistry 2020(04)
• [25].Synthesis and characterization functionalized poly(ether ether ketone)s with carboxylic groups[J]. Journal of Beijing Institute of Technology 2012(01)
• [26].Nanosized GdVO_4 powders synthesized by sol-gel method using different carboxylic acids[J]. Rare Metals 2018(07)
• [27].A novel application of horseradish peroxidase:Oxidation of alcohol ethoxylate to alkylether carboxylic acid[J]. Chinese Chemical Letters 2008(12)
• [28].Advances in self-assembly and regulation of aromatic carboxylic acid derivatives at HOPG interface[J]. Chinese Chemical Letters 2020(01)
• [29].Electrochemical sensor based on f-SWCNT and carboxylic group functionalized PEDOT for the sensitive determination of bisphenol A[J]. Chinese Chemical Letters 2014(04)
• [30].Design,synthesis and screening of some novel benzoxazole based 1,3,4-oxadiazoles as potential antimicrobial agents[J]. Chinese Chemical Letters 2013(07)

标签：放线菌论文;  次生代谢产物论文;  分离纯化论文;  抑菌活性论文;