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Effects of Co-inoculating Soil Yeast （Saccharomyces Cerevisiae and Saccharomyces Exiguus）with Bacteria （Bradyrhizobium Japonicum）on Growth,Nitrogen Fixation and Yield of Glycine Max（L.）

2020-12-09阅读(957)

Effects of Co-inoculating Soil Yeast （Saccharomyces Cerevisiae and Saccharomyces Exiguus）with Bacteria （Bradyrhizobium Japonicum）on Growth,Nitrogen Fixation and Yield of Glycine Max（L.）

论文摘要

土壤中植物大量营养素(氮、磷、钾)的有效性是影响作物产量的主要因素之一。这些矿物元素的有效性和形式因土壤类型的不同而有很大的差异。研究表明,通过为植物提供适当的微生物共生体,可以优化土壤营养对植物的有效补充。此外,土壤酵母能够溶解不可利用的磷和铁,并产生植物生长激素,这些激素可帮助植物生长和维持细菌活性。本研究旨在探讨共接种植物促生长细菌(PGPB)—慢生大豆根瘤菌(Bradyrhizobium japonicum)和植物促生长真菌—土壤酵母(Saccharomyces cerevisiae和Saccharomyces exiguus)对大豆(Glycine maxL.Merr)生长和产量的影响,特别是土壤酵母如何影响细菌的空气氮固定率和结瘤率。本研究以台湾292大豆品种为试验对象,采用完全随机实验设计:接种慢生大豆根瘤菌B.japonicum(T1;接种土壤酵母S.cerevisiae(T2;接种S.exiguus(T3);共接种土壤酵母S.cerevisiae和B.japonicum(T4);共接种S.exiguous及B.japonicum(T5)以及对照(T6),每个处理重复4次。结果表明,在大豆上共接种B.japonicum和S.cerevisiae以及共接种S.exiguous和B.japonicum,可明显改善幼苗生长初期的根和茎生长以及干物质累积。与B.jponicum单接种和未接种处理相比,大豆根瘤数、根瘤干重、根茎生长、大气氮固定效率、养分含量和种子产量均有改善。与双接种相比,单接种B.japonicum和单接种S.cerevisiae或S.exiguous对大豆幼苗期植株生长发育、叶片叶绿素含量、根瘤数、根瘤干重、大气氮固定效率、植株干重、植株磷、氮含量和蛋白质含量没有显著改善。结果表明,土壤酵母菌和硝化细菌的双重应用提高了大豆的生物化学和生理活性,促进了大豆的生长和生产力的提高。建议将土壤酵母和硝化细菌联合接种,作为提高大豆产量的新型生物肥料。

论文目录

  • 摘要
  • ABSTRACT
  • Chapter 1
  •   1 Background
  •     1.1 Soybean production and demand
  •     1.2 Fertilization problems in soybean production
  •     1.3 Importance of microbes in crop production
  •     1.4 Research advancements in bio-fertilizer use in crop production
  •     1.5 Aims and objectives of the study
  • Chapter 2
  •   2 Materials and methods
  •     2.1 Plant material
  •     2.2 Microbial strains used
  •     2.3 Experimental design
  •     2.4 Experimental site location
  •     2.5 Green house experiment
  •     2.6 Soil characterisation
  •       2.6.1 Soil physiochemical parameters
  •       2.6.2 Soil p H determination
  •       2.6.3 Soil elemental analysis
  •     2.7 Inoculum preparation and seed treatment
  •     2.8 Data collection
  •       2.8.1 Plant sampling
  •     2.8.1.1 Soybean germination test
  •     2.8.1.2 Root elongation bioassay
  •     2.8.1.3 Nodulation
  •     2.8.1.4 Soil microbiological analysis
  •     2.8.1.5 Shoots for collection of plant sap （root bleeding） for determination of atmospheric nitrogen （N2） fixation, dry matter and determination of nitrogen （N） and phosphorus （P） in soybean leaves
  •     2.8.1.6 Determination of chlorophyll content
  •     2.8.1.7 Seeds for determination of the nutritional value of harvested seed
  •     2.8.1.8 Grain yield
  •     2.8.1.9 Correlation analysis among traits
  •     2.9 Data analysis
  • CHAPTER 3
  •   3 Results
  •     3.1 Soybean initial growth and development
  •     3.2 Soybean nodulation
  •     3.3 Atmospheric nitrogen fixation
  •     3.4 Yield parameters
  • Chapter 4
  •   4 Discussion
  •     4.1 Soybean initial growth and development
  •       4.1.1 Seedling shoot analysis
  •     4.2 Soybean nodulation analysis
  •       4.2.1 Nodule number and nodule dry weight
  •     4.3 Chlorophyll content （SPAD Values） leaf area and percentage of total plantnitrogen derived from atmospheric fixation （%Ndfa）
  •     4.4 Microbial count
  •     4.5 Yield parameters
  • Chapter 5
  •   5 Conclusion
  • Chapter 6
  •   6 Recommendations
  • Chapter 7
  •   7 Acknowledgements
  • List of References

    • 文章来源

    类型: 硕士论文

    作者: Zveushe Obey Kudakwashe

    导师: Han Ying

    关键词: 尿素,大豆

    来源: 西南科技大学

    年度: 2019

    分类: 基础科学,农业科技

    专业: 生物学,农业基础科学,农艺学,农作物

    单位: 西南科技大学

    分类号: S565.1;S154.3

    总页数: 50

    文件大小: 3131K

    下载量: 14

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    Effects of Co-inoculating Soil Yeast （Saccharomyces Cerevisiae and Saccharomyces Exiguus）with Bacteria （Bradyrhizobium Japonicum）on Growth,Nitrogen Fixation and Yield of Glycine Max（L.）
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