Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins

论文摘要

The importance of immunoglobulin Y（IgY） as a specific antibody equivalent to mammalian immunoglobulin G（IgG） is well recognized. However, production of highly purified IgY is still difficult due to the lack of specific purification methods. In this study, adsorption behaviors of Ig Y on four mixed-mode resins with functional ligands of 4-mercatoethyl-pyridine（MEP）, 2-mercapto-1-methyli-midazole（MMI）, 5-aminobenzi-midazole（ABI） and tryptophan-5-aminobenzi-midazole（W-ABI） were evaluated. The results showed that high adsorption ratio were found at p H 6.0–7.0 with little adsorption under acidic conditions. The resin with ABI ligand was then used to separate IgY from immunized chicken serum. An efficient process with Ig Y purity of 95% and recovery of 90% was developed after optimization of loading and elution p H and injection volume. The biological activity of the purified Ig Y was fully maintained. These results indicated that mixed-mode chromatography with specially-designed ligands has great potential for the separation of Ig Y from crude feedstock.

论文目录

文章来源

类型: 期刊论文

作者: Yingdi Luo,Qilei Zhang,Shanjing Yao,Dongqiang Lin

来源: Chinese Journal of Chemical Engineering 2019年03期

年度: 2019

分类: 工程科技Ⅰ辑

专业: 化学,一般化学工业

单位: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University

基金: Supported by the National Natural Science Foundation of China(21776243,21476198),the International Science&Technology Cooperation Program of China(2015DFG42070)

分类号: O647.3;TQ937

页码: 514-518

总页数: 5

文件大小: 1273K

下载量: 6

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