论文摘要
Fourier-transform infrared（FTIR） spectroscopy has emerged as a viable alternative to biochemical and molecular biology techniques for bacterial typing with advantages such as short analysis time, low cost and laboratorial simplicity. In this study, synchrotron radiationbased FTIR（SR-FTIR） spectroscopy with higher spectral quality was successfully applied to type 16 foodborne pathogenic bacterial strains. Combined with principal component analysis（PCA） and hierarchical cluster analysis（HCA）, we found that the specific spectral region1300-1000 cm-1, which reflects the information of phosphate compounds and polysaccharides, can be used as the signature region to cluster the strains into groups similar with genetic taxonomic method. These findings demonstrated that FTIR spectra combined with HCA have a great potential in quickly typing bacteria depending on their biochemical signatures.
论文目录
文章来源
类型: 期刊论文
作者: Ya-Di Wang,Xue-Ling Li,Jun Hu,Jun-Hong Lü
来源: Nuclear Science and Techniques 2019年02期
年度: 2019
分类: 工程科技Ⅱ辑,工程科技Ⅰ辑,医药卫生科技
专业: 化学,轻工业手工业,预防医学与卫生学
单位: Division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology,Shanghai Institute of Applied Physics,Chinese Academy of Sciences (CAS),University of Chinese Academy of Sciences,Shanghai Center for Bioinformation Technology,Shanghai Industrial Technology Institute
基金: supported by the National Natural Science Foundation of China(Nos.U1732130 and 11474298),Key Research Program of Frontier Sciences of the Chinese Academy Sciences(No.QYZDJSSW-SLH019)
分类号: R155.5;O657.33
页码: 106-110
总页数: 5
文件大小: 908K
下载量: 9
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