Pre-incubated with BSA-complexed free fatty acids alters ER stress/autophagic gene expression by carboxylated multi-walled carbon nanotube exposure in THP-1 macrophages

论文摘要

Recently we found that multi-walled carbon nanotube（MWCNT）exposure alters the mRNA levels of endoplasmic reticulum（ER）stress/autophagic genes,but the impact of biological molecules on this response is unclear.Herein,we compared the different actions of carboxylated MWCNTs（c-MWCNTs）pre-incubated with bovine serum albumin（BSA）or BSA-complexed free fatty acid（denoted as FFA）on macrophages derived from THP-1 monocytes（denoted as THP-1 macrophages）.C-MWCNTs exhibited increased diameter and hydrodynamic size as well as decreased absolute zeta potential value after preincubation with BSA or FFA,which suggested a coating effect.Cytotoxicity or oxidative stress were not significantly induced after exposure to BSA-or FFA-coated c-MWCNTs.BSA-pre-incubated c-MWCNTs significantly enhanced the expression of the ER stress gene,DDIT3 and the autophagic genes,ATG5,BECN1,and PLIN2,but the mRNA levels of these genes was significantly decreased by FFA-pre-incubated c-MWCNTs.FFA-pre-incubated c-MWCNTs induced significantly higher lipid accumulation and interleukin-6（IL-6）protein level compared with BSA-p re-incubated c-MWCNTs,which suggested that FFA-p re-incubated c-MWCNTs may more effectively induce the formation of macrophage foam cells.Collectively,our data indicated that pre-incubation with FFA may influence c-MWCNT-induced ER stress/autophagic gene expression and foam cell formation in THP-1 macrophages.

论文目录

文章来源

类型: 期刊论文

作者: Qianyu Yang,Maolin Wang,Yongbing Sun,Shengming Peng,Yanhuai Ding,Yi Cao

来源: Chinese Chemical Letters 2019年06期

年度: 2019

分类: 工程科技Ⅰ辑,医药卫生科技

专业: 无机化工,材料科学,预防医学与卫生学

单位: Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education,Lab of Biochemistry,College of Chemistry,Xiangtan University,National Engineering Research Center for Solid Preparation Technology of Chinese Medicines,Jiangxi University of Traditional Chinese Medicines

基金: financially supported by the National Natural Science Foundation of China (No. 21707114),Scientific Research Foundation of Hunan Provincial Education Department (No. 17A205)

分类号: TQ127.11;TB383.1;R114

页码: 1224-1228

总页数: 5

文件大小: 818K

下载量: 10

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