Optimization of Coenzyme Q10Production Procedure from Rhodobacter sphaeroides Overexpressing UbiE

Optimization of Coenzyme Q10Production Procedure from Rhodobacter sphaeroides Overexpressing UbiE

论文摘要

In the present study, single factors including inoculation amount, fermentation temperature, fermentation duration and ratio of fermentation medium volume to total flask volume were optimized for enhancing the production of coenzyme Q10 from genetic engineered Rhodobacter sphaeroides overexpressing UbiE. The experimental results suggested that optimal single factors were: inoculation amount 2%, fermentation temperature 30 ℃, fermentation duration 48 h, and fermentation medium volume to total flask volume 80%. The present study will promote the large scale production of CoQ10 from microorganisms.

论文目录

  • Materials and Methods
  •   Growth conditions
  •   Effects of inoculation amount on the yield of CoQ10 from the screened genetic engineered strain
  •   Effects of fermentation temperature on the yield of CoQ10 from the screened genetic engineered strain
  •   Effects of fermentation duration on the yield of CoQ10 from the screened genetic engineered strain
  •   Effects of oxygen tension (fermentation medium volume to total flask volume) on the yield of CoQ10 from the screened genetic engineered strain
  • Results and Discussion
  •   Optimization of fermentation temperature
  •   Optimization of fermentation duration
  •   Optimization of inoculation amount
  •   Optimization of medium volume used in flasks
  • Conclusions
  • 文章来源

    类型: 期刊论文

    作者: Kuo TANG,Zhiping ZHAO,Li ZHANG,Zhenhui KANG

    来源: Agricultural Biotechnology 2019年05期

    年度: 2019

    分类: 农业科技,工程科技Ⅰ辑

    专业: 一般化学工业

    单位: School of Chemical Engineering,Sichuan University of Science & Engineering,School of Bioengineering,Sichuan University of Science &Engineering (Yibin Campus)

    基金: Supported by the Research Project of Sichuan University of Science&Engineering(2015RC27),Scientific Research Foundation of the Education Department of Sichuan Province(15ZA0222),Research Project of Meat Processing Key Laboratory of Sichuan Province(16R-27)

    分类号: TQ925

    DOI: 10.19759/j.cnki.2164-4993.2019.05.005

    页码: 19-20+25

    总页数: 3

    文件大小: 362K

    下载量: 13

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    Optimization of Coenzyme Q10Production Procedure from Rhodobacter sphaeroides Overexpressing UbiE
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