Astrocyte facilitated ramification remodeling of microglia via releasing TGF-β and gelatinous fiber traction

论文摘要

Background: Microglia are polymorphic corresponding to stimulus-dependent phenotypes,characterizing a wide range of functional outcomes.Although it is a well-known phenomenon that there were significant differences in morphology between microglia cells in vitro and in vivo, the biological mechanisms that regulate microglial branching and morphology are not well understood.Methods: In this study, the morphological study of microglia though immunocytochemical staining.And the morphological changes of microglia were quantized by the number of branches, the length of branches and the area of microglia.The role of astrocytes in the processes refinement of microglia was examined by pharmacologic astrocyte ablation strategy in hippocampus and cortex using stereotactic injection L-AAA.We studied astrocytes regulating microglial branching in a noncontact dependent manner by using astrocyte conditioned medium and transwell to culture microglia.To investigate the role of TGF-β in microglial branch remodeling, we blocked the TGF-β signaling pathway with TGF-β antibody.We studied astrocytes regulating microglial branching in a contact dependent manner by using astrocyte co-culture with microglia.Result: We found the brain microglia showed a more ramified phenotype, with a higher number of branches and length than in vitro.Ablation of astrocytes in cortex and hippocampus by L-AAA treatment results in decrease in the number of branches and increase in the cell bodies of microglia.The secretome of astrocyte promote the ramification remodeling of microglia.Blocking astrocytederived TGF-β inhibit microglial ramification remodeling.The co-culture of microglia and astrocytes can further promote the ramification remodeling of microglia.The refinement of the branches surrounded by glial fibrillary acidic proteins is similar to that of microglia in the brain.Conclusions: The results demonstrated that astrocyte regulate the microglial morphology by contact-dependent and non-contact-dependent patterns.Astrocytes-driven TGF-β play primary role in remodeling of the main branches of microglia.Refinement of microglial ramification depended on direct contact with astrocytes.These findings will help us better understand the physiological basis of glial cells and provide support for further research on glial cell-related neurological diseases.

论文目录

文章来源

类型: 国内会议

作者: 易赛妮,Jinqiang Zhang,Xue Jiang,Xiaofang Tang,Yue Zhang,Chenghong Xiao,Weike Jiang,Tao Zhou

来源: 2019中国生理学会学术年会暨张锡钧基金第十五届全国青年优秀生理学学术论文交流会及第十三届全国青年生理学工作者学术会议 2019-12-20

年度: 2019

分类: 基础科学,医药卫生科技

专业: 生物学,基础医学

单位: Guizhou University of Traditional Chinese Medicine,School of Life Science and Technology,Center for Informational Biology,University of Electronic Science and Technology of China

分类号: R338

DOI: 10.26914/c.cnkihy.2019.066276

页码: 246-247

总页数: 2

文件大小: 590k

下载量: 2

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