论文摘要
Noncoding RNAs（ncRNAs） can finely control the expression of target genes at the posttranscriptional level in prokaryotes. Regulatory small RNAs（sRNAs） designed to control target gene expression for applications in metabolic engineering and synthetic biology have been successfully developed and used. However, the effect on the heterologous expression of speciesor strain-specific ncRNAs in other bacterial strains remains poorly understood. In this work, a Pseudomonas stutzeri species-specific regulatory ncRNA, NfiS, which has been shown to play an important role in the response to oxidative stress as well as osmotic stress in P. stutzeri A1501, was cloned and transferred to the Escherichia coli strain Trans10. Recombinant NfiS-expressing E. coli, namely, Trans10-nfiS,exhibited significant enhancement of tolerance to oxidative stress. To map the possible gene regulatory networks mediated by NfiS in E. coli under oxidative stress, a microarray assay was performed to delineate the transcriptomic differences between Trans10-nfiS andwild-type E. coli under H2 O2 shock treatment conditions. In all, 1184 genes were found to be significantly altered, and these genes were divided into mainly five functional categories: stress response, regulation, metabolism related, transport or membrane protein and unknown function. Our results suggest that the P. stutzeri species-specific ncRNA NfiS acts as a regulator that integrates adaptation to H2 O2 with other cellular stress responses and helps protect E. coli cells against oxidative damage.
论文目录
文章来源
类型: 国内会议
作者: Guihua Hu,Tao Hu,Yuhua Zhan,Wei Lu,Min Lin,Yunhong Huang,Yongliang Yan
来源: 中国生物工程学会第十三届学术年会暨2019年全国生物技术大会 2019-11-09
年度: 2019
分类: 基础科学
专业: 生物学,生物学
单位: College of Life Sciences, Jiangxi Normal UniversityBiotechnology Research Institute, Chinese Academy of Agricultural Sciences
分类号: Q933
DOI: 10.26914/c.cnkihy.2019.026868
页码: 341
总页数: 1
文件大小: 190k
下载量: 6