芽抱杆菌论文_陈秉梅,许小平,侯志国,李忠琴,阮文兵

导读:本文包含了芽抱杆菌论文开题报告文献综述、选题提纲参考文献及外文文献翻译，主要关键词:杆菌,芽孢,基质,微生物,作用,论文,浓香型。

芽抱杆菌论文文献综述

陈秉梅,许小平,侯志国,李忠琴,阮文兵^[1]（2011）在《产(R)-α-基苯乙酸的芽抱杆菌B26的鉴定与诱变育种(英文)》一文中研究指出A bacterium strain B26 capable of producing(R)-α-hydroxyphenylacetic acid [(R)-HPA](yield,47.5%;enantiomeric excess,99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as Bacillus sp.B26 by 16S rDNA(ribosomal DNA) sequencing.Phylogenic analysis showed that the strain was most similar to Bacillus sp.enrichment culture clone SYW5(FJ601635.1) and Bacillus cereus strain FM-4(EU794727.1).Efforts were made to further improve HPA-production and PGA-tolerance by UV irradiation and UV-LiCl cooperative mutagenesis.Among viable mutants,B.sp.UV-38 and B.sp.ULi-11 exhibited better productivities than the wild type.Comparisons of HPA production and time course among wild strain and two mutants showed that B.sp.ULi-11 was more competent than B.sp.UV-38.HPA production was increased by 39.1% with B.sp.ULi-11(yield,65.4%) compared to that with B.sp.B26(yield,47.0%) when cultured in fermentation broth(pH 7.2) at 32℃ with an agitation speed of 180 r·min-1 and PGA final concentration of 15 mmol·L-1 for 25 h.(本文来源于《Chinese Journal of Chemical Engineering》期刊2011年04期）

杨革,张营,白艳芬^[2]（2011）在《蜡状芽抱杆菌壬基苯酚降解酶的分离纯化及其酶学性质(英文)》一文中研究指出<Abstract>An extracellular NP-degrading enzyme secreted by Bacillus cereus.Frankland was purified to homogeneity by a combination of ammonium sulfate precipitation,Phenyl-Sepharose hydrophobic-interaction chromatography and DEAE anion-exchange chromatography.On SDS(sodium dodecyl sulfate)-polyacrylamide gel electrophoresis analysis,the purified enzyme showed a relative molecular mass of 58.3 kDa.The depolymerzation of subunits was accompanied with the loss of NP-degrading enzyme activity,and removing denaturing factors by dialysis could restore the dimer structure and enzymatic activity.The enzyme had an isoelectric point of 5.5 and an optimal temperature of 60℃,and was the most active at pH 6.0.The enzymatic activity was stable at pH 4-8 and inhibited by Cu2+.TenN-terminal amino acids were determined to be ASVNSIKIGY,demonstrating that the purified enzyme was a novel one.The hydrolysis pattern of the purified enzyme indicated that the NP-degrading enzyme was an endo NP-degrading enzyme.The extraordinary thermo-stability provided the enzyme with a good prospect to be used as a new tool for clean-production process for textile industry.(本文来源于《Chinese Journal of Chemical Engineering》期刊2011年04期）

吴水清^[3]（1993）在《梭状芽抱杆菌L—Ⅱ基质范围的代谢研究》一文中研究指出对梭状芽孢杆菌L—Ⅱ的含碳基质范围的研究表明：该菌株能利用乙酸、丙酸、丁酸、乙醇和丙醇。以乙醇和乙酸为底物时，主要产物是丁酸和己酸。以乙醇和丙酸为底物时，主要产物是戊酸和庚酸。以乙酸和丙醇为底物时，主要产物是丙酸、戊酸、丁酸和己酸。该菌的丙酸生物合成途径与克氏梭菌基本一致，适用于科研和浓香型酒生产。（益平）(本文来源于《酿酒科技》期刊1993年04期）

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<Abstract>An extracellular NP-degrading enzyme secreted by Bacillus cereus.Frankland was purified to homogeneity by a combination of ammonium sulfate precipitation,Phenyl-Sepharose hydrophobic-interaction chromatography and DEAE anion-exchange chromatography.On SDS(sodium dodecyl sulfate)-polyacrylamide gel electrophoresis analysis,the purified enzyme showed a relative molecular mass of 58.3 kDa.The depolymerzation of subunits was accompanied with the loss of NP-degrading enzyme activity,and removing denaturing factors by dialysis could restore the dimer structure and enzymatic activity.The enzyme had an isoelectric point of 5.5 and an optimal temperature of 60℃,and was the most active at pH 6.0.The enzymatic activity was stable at pH 4-8 and inhibited by Cu2+.TenN-terminal amino acids were determined to be ASVNSIKIGY,demonstrating that the purified enzyme was a novel one.The hydrolysis pattern of the purified enzyme indicated that the NP-degrading enzyme was an endo NP-degrading enzyme.The extraordinary thermo-stability provided the enzyme with a good prospect to be used as a new tool for clean-production process for textile industry.

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