适用于Nimble Cloning系统的pCambia载体改造

论文摘要

为了适用于Nimble Cloning这种新型的DNA分子克隆技术,本研究以pCambia1304载体作为改造对象,采用突变碱基的方式去除载体中的5个Sfi I酶切位点,并且对改造后的载体在农杆菌中的稳定性以及在植物中的表达进行分析,结果表明5个Sfi I位点突变对载体的稳定性和表达没有影响。本研究为Nimble Cloning系统提供了配套载体,也为其他pCambia系列载体改造成Nimble Cloning系统载体提供了模板和参考。

论文目录

文章来源

类型: 期刊论文

作者: 曾妍静,沈文涛,庹德财,黎小瑛,言普,周鹏

关键词: 分子克隆技术,系统,载体,突变,酶切位点

来源: 生命科学研究 2019年04期

年度: 2019

分类: 基础科学

专业: 生物学

单位: 海南大学热带农林学院,中国热带农业科学院热带生物技术研究所

基金: 中国热带农业科学院基本科研业务费专项(1630052018004,1630052019004)

分类号: Q782

DOI: 10.16605/j.cnki.1007-7847.2019.04.004

页码: 276-280

总页数: 5

文件大小: 1830K

下载量: 94

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标签：分子克隆技术论文;  系统论文;  载体论文;  突变论文;  酶切位点论文;