Design, mechanism, delivery and therapeutics of canonical and Dicer-substrate siRNA

论文摘要

Upon the discovery of RNA interference（RNAi）,canonical small interfering RNA（si RNA） has been recognized to trigger sequence-specific gene silencing. Despite the benefits of si RNAs as potential new drugs,there are obstacles still to be overcome,including off-target effects and immune stimulation. More recently,Dicer substrate si RNA（Dsi RNA） has been introduced as an alternative to si RNA. Similarly,it also is proving to be potent and target-specific,while rendering less immune stimulation. Dsi RNA is 25–30 nucleotides in length,and is further cleaved and processed by the Dicer enzyme. As with si RNA,it is crucial to design and develop a stable,safe,and efficient system for the delivery of Dsi RNA into the cytoplasm of targeted cells. Several polymeric nanoparticle systems have been well established to load Dsi RNA for in vitro and in vivo delivery,thereby overcoming a major hurdle in the therapeutic uses of Dsi RNA. The present review focuses on a comparison of si RNA and Dsi RNA on the basis of their design,mechanism,in vitro and in vivo delivery,and therapeutics.

论文目录

文章来源

类型: 期刊论文

作者: Maria Abdul Ghafoor Raja,Haliza Katas,Muhammad Wahab Amjad

来源: Asian Journal of Pharmaceutical Sciences 2019年05期

年度: 2019

分类: 医药卫生科技,工程科技Ⅰ辑

专业: 有机化工,药学

单位: Department of Pharmaceutics,Faculty of Pharmacy,Northern Border University,Centre for Drug Delivery Research,Faculty of Pharmacy,Universiti Kebangsaan Malaysia

基金: financial support received from Centre of Research and Instrumentation (CRIM),Universiti Kebangsaan Malaysia

分类号: R943

页码: 497-510

总页数: 14

文件大小: 1674K

下载量: 13

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