论文摘要
A new α-fetoprotein-MIP（AFP-MIP） immunosensor based on glass carbon electrode（GCE） modified with polythionine（PTh） and gold nanoparticles（AuNPs） was successfully fabricated for sensitive detection ofα-fetoprotein（AFP）. Through controlling electropolymerization, A "polydopamine（PDA）-AFP" complex was achieved applying AFP as template and dopamine（DA） as imprinted monomers. After elution, the specific cavities can adsorb the target molecules. Using differential pulse voltammetry（DPV） detection,the peak current decreased with the increase in concentration of AFP, and the linear response range of the AFP-MIP immunosensor was from 0.001 ng/mL to 800 ng/mL with the detection limit as low as0.8138 pg/mL. The MIP immunosensor could become a new promising method for the detection of AFP.Furthermore, this MIP sensor was demonstrated in testing AFP in human serum samples with satisfactory results.
论文目录
文章来源
类型: 期刊论文
作者: Yuxuan Lai,Chuanxiang Zhang,Yan Deng,Gaojian Yang,Song Li,Congli Tang,Nongyue He
来源: Chinese Chemical Letters 2019年01期
年度: 2019
分类: 工程科技Ⅰ辑,信息科技
专业: 化学,自动化技术
单位: Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology,College of Packing and Materials Engineering, Hunan University of Technology,State Key Laboratory of Bioelectronics, Southeast University
基金: the National Natural Science Foundation of China (Nos. 61471168,61571187),China Postdoctoral Science Foundation (No. 2016T90403),Postdoctoral Science Foundation of Jiangsu Province (No. 1601021A),the Natural Science Foundation of Hunan Province (No. 2017JJ209),Hunan Key Research Project (No. 2017SK2174) for the financial supports
分类号: O631.3;TP212
页码: 160-162
总页数: 3
文件大小: 781K
下载量: 9
相关论文文献
- [1].Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma[J]. World Journal of Gastroenterology 2017(19)
- [2].Prognostic role of alpha-fetoprotein response after hepatocellular carcinoma resection[J]. World Journal of Clinical Cases 2018(06)
- [3].Expression of miRNA-29b and its clinical significances in primary hepatic carcinoma[J]. China Medical Abstracts(Internal Medicine) 2013(02)
- [4].Association between serum alpha-fetoprotein levels and fatty liver disease: A cross-sectional study[J]. World Journal of Gastroenterology 2014(33)
- [5].Contribution of alpha-fetoprotein in liver transplantation for hepatocellular carcinoma[J]. World Journal of Hepatology 2016(21)
- [6].Assessment of the correlation between serum prolidase and alpha-fetoprotein levels in patients with hepatocellular carcinoma[J]. World Journal of Gastroenterology 2015(22)
- [7].Elevated serum alpha fetoprotein levels promote pathological progression of hepatocellular carcinoma[J]. World Journal of Gastroenterology 2011(41)
- [8].Alpha-fetoprotein specific CD4 and CD8 T cell responses in patients with hepatocellular carcinoma[J]. World Journal of Hepatology 2010(07)
- [9].Diagnostic value of PIVKA-Ⅱ and alpha-fetoprotein in hepatitis B virus-associated hepatocellular carcinoma[J]. World Journal of Gastroenterology 2015(13)
- [10].Clinical diagnosis and treatment of alpha-fetoprotein-negative small hepatic lesions[J]. Chinese Journal of Cancer Research 2013(04)
- [11].Clinicopathologic characteristics of intrahepatic cholangiocarcinoma in patients with positive serum a-fetoprotein[J]. World Journal of Gastroenterology 2008(14)
- [12].Using receiver operating characteristic curves to evaluate the diagnostic value of the combination of multislice spiral CT and alpha-fetoprotein levels for small hepatocellular carcinoma in cirrhotic patients[J]. Hepatobiliary & Pancreatic Diseases International 2017(03)
- [13].Cyst and tumor of liver[J]. China Medical Abstracts(Internal Medicine) 2008(01)
- [14].Protein induced by vitamin K absence or antagonist-Ⅱ versus alpha-fetoprotein in the diagnosis of hepatocellular carcinoma: A systematic review with meta-analysis[J]. Hepatobiliary & Pancreatic Diseases International 2018(06)
- [15].Possible connection between elevated serum α-fetoprotein and placental necrosis during pregnancy: A case report and review of literature[J]. World Journal of Clinical Cases 2018(13)
- [16].Diagnostic value of gamma-glutamyltransferase/aspartate aminotransferase ratio, protein induced by vitamin K absence or antagonist II, and alpha-fetoprotein in hepatitis B virus-related hepatocellular carcinoma[J]. World Journal of Gastroenterology 2019(36)
- [17].Survival outcomes of liver transplantation for hepatocellular carcinoma in patients with normal, high and very high preoperative alpha-fetoprotein levels[J]. World Journal of Hepatology 2018(02)
- [18].Alpha-fetoprotein:A controversial prognostic biomarker for small hepatocellular carcinoma[J]. World Journal of Gastroenterology 2013(03)
- [19].Clinical Significance of a Rise in AFP in Lung Adenocarcinoma Patients with Liver Metastasis:One Case Report and Literatures Review[J]. Chinese Journal of Clinical Oncology 2008(03)
- [20].Value of α-fetoprotein in association with clinicopathological features of hepatocellular carcinoma[J]. World Journal of Gastroenterology 2013(11)
- [21].Primary Endodermal Sinus Tumor in the Posterior Cranial Fossa:Clinical Analysis of 7 Cases[J]. Chinese Medical Sciences Journal 2013(04)
- [22].Highlights for α-fetoprotein in determining prognosis and treatment monitoring for hepatocellular carcinoma[J]. World Journal of Gastroenterology 2012(48)
- [23].Clinicopathological features of alpha-fetoprotein producing early gastric cancer with enteroblastic differentiation[J]. World Journal of Gastroenterology 2016(36)
- [24].Neoadjuvant sorafenib combined with gemcitabine plus oxaliplatin in advanced hepatocellular carcinoma[J]. World Journal of Gastroenterology 2011(17)
- [25].Alpha-fetoprotein before and after pegylated interferon therapy for predicting hepatocellular carcinoma development[J]. World Journal of Hepatology 2015(19)
- [26].Changes of serum alpha-fetoprotein and alpha-fetoprotein-L3 after hepatectomy for hepatocellular carcinoma: prognostic significance[J]. Hepatobiliary & Pancreatic Diseases International 2012(06)
- [27].Diagnosis and treatment of primary embryonal carcinoma in pineal region:report of two cases and literatures review[J]. China Medical Abstracts(Surgery) 2011(03)
- [28].Establishment of a pattern recognition metabolomics model for the diagnosis of hepatocellular carcinoma[J]. World Journal of Gastroenterology 2020(31)
- [29].Survivals after liver transplantation for hepatocellular carcinoma:Granular data for a better allocation process?[J]. Hepatobiliary & Pancreatic Diseases International 2018(04)
- [30].An isolate alpha-fetoprotein producing gastric cancer liver metastasis emerged in a patient previously affected by radiation induced liver disease[J]. World Journal of Hepatology 2013(07)