Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers

论文摘要

A simple,convenient,and highly sensitive bio-interface for graphene field-effect transistors（GFETs） based on multifunctional nano-denatured bovine serum albumin（nano-dBSA） functionalization was developed to target cancer bio-markers.The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface.The formed nano-d BSA film served as the cross-linker to immobilize monoclonal antibody against car-cinoembryonic antigen（anti-CEA mAb） on the graphene channel activated by EDC and Sulfo-NHS.The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing.The improved GFETbiosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL-1.The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction,indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82×10-10M.The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing,nanomedicine,and drug delivery.

论文目录

文章来源

类型: 期刊论文

作者: Lin Zhou,Kun Wang,Hao Sun,Simin Zhao,Xianfeng Chen,Dahong Qian,Hongju Mao,Jianlong Zhao

来源: Nano-Micro Letters 2019年02期

年度: 2019

分类: 工程科技Ⅰ辑,信息科技

专业: 化学,自动化技术

单位: State Key Laboratory of Transducer Technology,Key Laboratory of Terahertz Solid-State Technology,Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Sciences,School of Biomedical Engineering,Shanghai Jiao Tong University,School of Electronic Engineering,Bangor University

基金: the support of grants from the National Key R&D Program of China(Nos.2018YFA0108202 and 2017YFA0205300),the National Natural Science Foundation of China(Nos.61571429,61801464,61801465,and 81471748),the STS Project of the Chinese Academy of Sciences(NO.KFJ-STS-SCYD-120),the Science and Technology Commission of Shanghai Municipality(Nos.16410711800 and 14391901900)

分类号: TP212.3;O657.1

页码: 24-36

总页数: 13

文件大小: 878K

下载量: 32

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