SVB1 and SVB2 Regulate ABA Response and Plant Morphology in Arabidopsis Thaliana

SVB1 and SVB2 Regulate ABA Response and Plant Morphology in Arabidopsis Thaliana

论文摘要

脱落酸(Abscisic acid,ABA)是调控植物生长发育及植物对外界环境响应的重要胁迫激素。在拟南芥中越来越多基因已被报道参与调控ABA信号转导和胁迫应答,但还有很多ABA响应基因的功能尚未得到研究。在本文中,我们发现拟南芥At4g24130(SVB1)和At1g56580(SVB2)是ABA响应基因。定量RT-PCR结果显示ABA处理后SVB1和SVB2的表达量显著增加。原生质体转染实验表明SVB1和SVB2定位在除了核以外的整个细胞中。RT-PCR结果表明SVB1和SVB2两个基因在成熟的花中表达量都很高,但是SVB2也在莲座叶、荚果和茎等其他组织器官中表达。种子萌发实验表明SVB2过表达植株以及svb1单突变体和svb1 svb2双突变体对ABA敏感性增加。但svb2单突变体种子较野生型萌发早,且对ABA敏感性降低。绿苗率实验结果表明35S:SVB2转基因植株和svb1 svb2双突变体对ABA敏感性增加。盐处理实验表明SVBs在调控植物的盐胁迫响应中具有冗余功能。表型观察发现SVB2过表达植株和野生型相比种子更大,根长也更长。显微观察发现svb2单突变体表皮毛数量减少,并且只有1和2个分支的表皮毛的数量明显增多。综上所述,我们的研究结果表明SVBs参与调控拟南芥形态发育及其对ABA的响应。

论文目录

  • Abstract
  • 摘要
  • Introduction
  •   1.Abscisic Acid (ABA)
  •     1.1 ABA signaling in plants
  •     1.2 ABA binding proteins and receptors
  •     1.3 Role of ABA in stress signaling
  •   2.ABA and abiotic stress tolerance
  •     2.1 ABA and salt tolarance
  •     2.2 ABA and drought tolarance
  •     2.3 Effect of ABA on seed germination and root elongation
  •   3.SVB (Smaller with Variable Branches)
  •   4.Purpose and significance of this study
  • Materials and methods
  •   1.Experimental materials
  •     1.1 Plant materials
  •     1.2 Bacterium strains and plasmids
  •     1.3 Reagents and kits used
  •     1.4 List of Conventional reagents used
  •     1.5 Restriction enzymes
  •     1.6 Preparation of growth medium
  •     1.7 Preparation of Antibiotics and buffers
  •   2.Primers design and synthesis
  •   3.Equipments
  •   4.Experimental Methods
  •     4.1 Seed sowing and vernalization
  •     4.2 Bioinformatics analysis
  •     4.3 Extraction of plant RNA and cDNA synthesis
  •     4.4 Cloning and vector contruction
  •     4.5 DNA extraction from Gel
  •     4.6 Enzyme digestion
  •     4.7 Transformation of Agrobacterium tumefaceium competent cells
  •     4.8 Plant transformation and transgenic plant selection
  •     4.9 Protoplast transfection assay
  •   5.Hormone treatment
  •     5.1 Germination assay
  •     5.2 Root Elongation assay
  •   6.Salinity stress
  •   7.Drought stress
  •   8.Morphological analysis
  •     8.1 Trichomes
  •     8.2 Seed size
  • Results
  •   1.SVB1 and SVB2 are ABA responsive genes
  •     1.1 Cloning of SVBs
  •   2.Subcellular localization of SVB1 and SVB2
  •     2.1 Construction of pUC19-GFP-SVB1 and pUC19-GFP-SVB2
  •     2.2 SVB1 and SVB2 are located in the entire cell except nucleus
  •   3.Expression pattern of SVB1 and SVB2
  •   4.SVB1 and SVB2 transgenic plants
  •     4.1 SVB1 and SVB2 overexpression plants
  •     4.2 SVB1 and SVB2 mutant plants
  •   5.Hormone Treatment
  •     5.1 Effect of ABA on seed germination rate of SVBs
  •     5.2 Effect of ABA on Cotyledon greening rate of SVBs
  •     5.3 Effect of drought stress on SVBs
  •     5.4 Effect of salt stress on SVBs
  •   6.Effect of ABA on SVBs root elongation
  •   7.Effect of NaCl on SVBs root elongation
  •   8.Effect of svb2 on trichome morphogenesis
  •     8.1 Effect of svb2 on trichome numbers
  •     8.2 Effect of svb2 on trichome branch number
  •   9.Effect of35S:SVB2 on seeds size
  • Discussion
  • Conclusion
  • References
  • List of Abbreviations
  • Acknowledgements
  • Publications
  • 文章来源

    类型: 硕士论文

    作者: Sajjad Ahmed

    导师: Wang Shucai

    关键词: 脱落酸,拟南芥,绿苗率,表皮毛

    来源: 东北师范大学

    年度: 2019

    分类: 基础科学

    专业: 生物学

    单位: 东北师范大学

    分类号: Q945

    总页数: 65

    文件大小: 7465K

    下载量: 21

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    SVB1 and SVB2 Regulate ABA Response and Plant Morphology in Arabidopsis Thaliana
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