论文摘要
We report the carboxylated C60 improved the survival and quality of boar sperm during liquid storage at 4 °C and thus propose the use of carboxylated C60 as a novel antioxidant semen extender supplement. Our results demonstrated that the sperm treated with 2 μg mL-1 carboxylated C60 had higher motility than the control group（58.6% and 35.4%, respectively; P ? 0.05）. Moreover, after incubation with carboxylated C60 for 10 days, acrosome integrity and mitochondrial activity of sperm increased by 18.1% and 34%, respectively, compared with that in the control group. Similarly, the antioxidation abilities and adenosine triphosphate levels in boar sperm treated with carboxylated C 60 significantly increased（P ? 0.05） compared with those in the control group. The presence of carboxylated C60 in semen extender increases sperm motility probably by suppressing reactive oxygen species（ROS） toxicity damage. Interestingly, carboxylated C60 could protect boar sperm from oxidative stress and energy deficiency by inhibiting the ROS-induced protein dephosphorylation via the cAMP-PKA signaling pathway. In addition, the safety of carboxylated C60 as an alternative antioxidant was also comprehensively evaluated by assessing the mean litter size and number of live o spring in the carboxylated C60 treatment group. Our findings confirm carboxylated C60 as a novel antioxidant agent and suggest its use as a semen extender supplement for assisted reproductive technology in domestic animals.
论文目录
文章来源
类型: 期刊论文
作者: Xinhong Li,Lirui Wang,Huan Liu,Jieli Fu,Linqing Zhen,Yuhua Li,Yaozhong Zhang,Yafei Zhang
来源: Nano-Micro Letters 2019年04期
年度: 2019
分类: 工程科技Ⅰ辑,农业科技
专业: 化学,畜牧与动物医学
单位: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University,Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Centre for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University,Department of Electrical and Computer Engineering, Michigan State University,Key Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro/Nano Electronics, School of Electronics, Information and Electrical Engineering, Shanghai Jiao Tong University
基金: supported by the National Natural Science Foundation of China (No. 31772594),the Special Fund for Agro-scientific Research in the Public Interest of China (No. 200903056),the Key Project of Shanghai Municipal Agricultural Commission of China (2014-2-5)
分类号: S828.3;O613.71
页码: 817-833
总页数: 17
文件大小: 2009K
下载量: 17
相关论文文献
- [1].Optimal analysis conditions for sperm motility parameters with a CASA system in a passerine bird, Passer montanus[J]. Avian Research 2019(04)
- [2].PIWI/piRNA Found to Play a Critical Role in Translation Activation during Sperm Formation[J]. Bulletin of the Chinese Academy of Sciences 2019(04)
- [3].Sperm collection in Black?legged Kittiwakes and characterization of sperm velocity and morphology[J]. Avian Research 2019(01)
- [4].HLA antigens and anti-sperm antibody production in Iranian vasectomized men[J]. The Journal of Biomedical Research 2015(01)
- [5].Use of hyaluronic acid for sperm immobilisation and selection before intracytoplasmic sperm injection:A systematic review and meta-analysis[J]. World Journal of Obstetrics and Gynecology 2015(04)
- [6].Effect of Wuziyanzong Pill on sperm quality and calcium ion content in oligoasthenospermia rats[J]. Journal of Traditional Chinese Medicine 2012(04)
- [7].Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm[J]. Journal of Animal Science and Biotechnology 2017(02)
- [8].Subspecific variation in sperm morphology and performance in the Long-tailed Finch (Poephila acuticauda)[J]. Avian Research 2015(04)
- [9].Study on sperm damage caused by trichloroethylene in male rats[J]. China Medical Abstracts(Internal Medicine) 2014(01)
- [10].Impact of external factors on sperm motility of Sepiella maindroni[J]. Chinese Journal of Oceanology and Limnology 2011(01)
- [11].Evaluation of sperm mitochondrial function using rh123/PI dual fluorescent staining in asthenospermia and oligoasthenozoospermia[J]. Journal of Biomedical Research 2010(05)
- [12].Knockdown of sAC affects sperm hyperactivation by cAMP-signaling pathway in male rat (Rattus norvegicus)[J]. Chinese Science Bulletin 2013(26)
- [13].Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility[J]. Journal of Animal Science and Biotechnology 2020(01)
- [14].Combination of Korean Red Ginseng Extract and Hydrogen-Rich Water Improves Spermatogenesis and Sperm Motility in Male Mice[J]. Chinese Journal of Integrative Medicine 2020(05)
- [15].Cryopreservation of strip spawned sperm using programmable freezing technique in the blue mussel Mytilus galloprovincialis[J]. Journal of Oceanology and Limnology 2018(06)
- [16].Effects of Clindamycin on Sperm Function in Mice[J]. Animal Husbandry and Feed Science 2010(Z3)
- [17].Single and double layer centrifugation improve the quality of cryopreserved bovine sperm from poor quality ejaculates[J]. Journal of Animal Science and Biotechnology 2017(01)
- [18].RNA Interference-Mediated Downregulation of sAC Gene Inhibits Sperm Hyperactivation in Male Rats(Rattus norvegicus)[J]. Journal of Integrative Agriculture 2014(02)
- [19].Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation[J]. Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012(01)
- [20].Epigenetic reprogramming of embryos derived from sperm frozen at-20℃[J]. Science China(Life Sciences) 2012(04)
- [21].Delivery of CatSper2 siRNA into Rat Sperms by Electroporation Repressed Ca~(2+) Influx During Sperm Hyperactivation[J]. Agricultural Sciences in China 2011(12)
- [22].Cistanchetubulosa(Schenk)R.Wight amelioratesbisphenol A-induced testicular and sperm toxicity in rats through steroidogenesis and redox pathways[J]. 中国药理学与毒理学杂志 2015(S1)
- [23].MSG is involved in sperm gelatinolytic activity in the prawn,Macrobrachium rosenbergii[J]. Chinese Science Bulletin 2013(18)
- [24].Manipulation of bicarbonate concentration in sperm capacitation media improves in vitro fertilisation output in porcine species[J]. Journal of Animal Science and Biotechnology 2019(02)
- [25].Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs[J]. Journal of Animal Science and Biotechnology 2017(03)
- [26].Antioxidative Protective Effect of Icariin on the FeSO_4/H_2O_2-damaged Human Sperm Based on Confocal Raman Micro-spectroscopy[J]. Journal of Huazhong University of Science and Technology(Medical Sciences) 2014(05)
- [27].Effects of the crude extract of Polygala tenuifolia Willd on human sperm in vitro[J]. Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011(06)
- [28].Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus[J]. Chinese Journal of Oceanology and Limnology 2016(04)
- [29].Semen Quality Assessment of Suyou Ⅰ Duck[J]. Animal Husbandry and Feed Science 2017(05)
- [30].Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation[J]. Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013(05)