<正>The Origin Recognition Complex （ORC） consists of six highly conserved subunits, Orc1-6.Despite the conservation in protein sequence, ORC from different species have divergent DNA binding specificities ranging from an absolute requirement for a specific sequence in Saccharomyces cerevisiae to no known sequence requirement in human. Yet, ORC from all species bind DNA to mark the sites for DNA replication initiation. Here, we have determined the cryo-EM structure of S. cerevisiae ORC bound to a 72-bp origin DNA containing the ARS consensus sequence （ACS） and B1 element at an overall resolution of 3.0?. In this structure,
类型: 国内会议
作者: Ning-ning Li,Wai Hei Lam,Yuan-liang Zhai,Jia-xuan Cheng,Erchao Cheng,Yong-qian Zhao,Bik-Kwoon Tye,Ning Gao
来源: 第六届全国冷冻电子显微学与结构生物学专题研讨会 2019-06-16
年度: 2019
分类: 基础科学
专业: 生物学
单位: State Key Laboratory of Membrane Biology, Peking-Tsinghua Center for Life Sciences, School of Life Sciences, Peking University,Division of Life Science, The Hong Kong University of Science and Technology
分类号: Q617
DOI: 10.26914/c.cnkihy.2019.038807
页码: 63
总页数: 1
文件大小: 144k
下载量: 4
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