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A comparison of fish tissue mercury concentrations from homogenized fillet and nonlethal biopsy plugs

论文摘要

The use of biopsy plugs to sample fish muscle tissue for mercury analyses is a viable alternative to lethal sampling; however, the practice has yet to be widely implemented in routine monitoring due to concerns about variability of mercury concentrations in fish muscle tissues. Here we examine distribution of mercury in fillets of four fish species（Walleye, Northern Pike, Smallmouth Bass and Lake Trout）, suitability of left/right side of fillet for biopsy sampling, and appropriateness of re-using a biopsy punch. The results showed that average mercury concentrations in left and right fillets of fish are similar.Mercury concentrations in biopsy plug samples, taken from the anterior dorsal area of the fish fillet, were statistically equivalent to the mercury concentrations in homogenized fillets. There was no discernible cross contamination between samples when a biopsy punch was reused after washing in hot soapy water, and as such, biopsy punches can be recycled during sampling to reduce the sampling cost. If a tissue mass collected from a specific site on the fillet is insufficient, then we suggest sampling corresponding locations on the other fillet rather than sampling two adjacent sites on one fillet to obtain more tissue. The results presented here can improve the accuracy of fillet biopsy plug sampling,minimize fish mortality for mercury monitoring, and reduce labor and material costs in monitoring programs.

论文目录

  • Introduction
  • 1. Materials and methods
  •   1.1. Sample collection
  •   1.2. Fillet and biopsy plug sampling
  •   1.3. Left and right fillet comparison
  •   1.4. Homogenized fillet and biopsy plug comparison
  •   1.5. Left and right biopsy plug sample comparison
  •   1.6. Re-use of biopsy punch
  •   1.7. Analytical methods
  •   1.8. Statistical analysis
  • 2. Results
  •   2.1. Left and right fillet comparison
  •   2.2. Homogenized fillet and biopsy plug comparison
  •   2.3. Left and right biopsy plug comparison
  •   2.4. Re-use of biopsy punch
  • 3. Discussion
  •   3.1. Spatial distribution of fillet mercury
  •   3.2. Biopsy method implementation
  • Conflict of interest
  • Appendix A.Supplementary data

    • 文章来源

    类型: 期刊论文

    作者: Alexis Knight,Satyendra P.Bhavsar,Brian A.Branfireun,Peter Drouin,Ram Prashad,Steve Petro,Moustapha Oke

    来源: Journal of Environmental Sciences 2019年06期

    年度: 2019

    分类: 工程科技Ⅰ辑

    专业: 环境科学与资源利用

    单位: Department of Biology and Centre for Environment and Sustainability, Western University

    分类号: X835

    页码: 137-145

    总页数: 9

    文件大小: 728K

    下载量: 14

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